Laser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection

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dc.contributor.authorZhao L.
dc.contributor.authorRosati G.
dc.contributor.authorPiper A.
dc.contributor.authorde Carvalho Castro e Silva C.
dc.contributor.authorHu L.
dc.contributor.authorYang Q.
dc.contributor.authorDella Pelle F.
dc.contributor.authorAlvarez-Diduk R.R.
dc.contributor.authorMerkoci A.
dc.date.accessioned2024-03-12T19:12:31Z
dc.date.available2024-03-12T19:12:31Z
dc.date.issued2023
dc.description.abstract© 2023 The Authors. Published by American Chemical Society.Graphene-based materials are of interest in electrochemical biosensing due to their unique properties, such as high surface areas, unique electrochemical properties, and biocompatibility. However, the scalable production of graphene electrodes remains a challenge; it is typically slow, expensive, and inefficient. Herein, we reported a simple, fast, and maskless method for large-scale, low-cost reduced graphene oxide electrode fabrication; using direct writing (laser scribing and inkjet printing) coupled with a stamp-transferring method. In this process, graphene oxide is simultaneously reduced and patterned with a laser, before being press-stamped onto polyester sheets. The transferred electrodes were characterized by SEM, XPS, Raman, and electrochemical methods. The biosensing utility of the electrodes was demonstrated by developing an electrochemical test for Escherichia coli. These biosensors exhibited a wide dynamic range (917-2.1 × 107 CFU/mL) of low limits of detection (283 CFU/mL) using just 5 μL of sample. The test was also verified in spiked artificial urine, and the sensor was integrated into a portable wireless system driven and measured by a smartphone. This work demonstrates the potential to use these biosensors for real-world, point-of-care applications. Hypothetically, the devices are suitable for the detection of other pathogenic bacteria.
dc.identifier.doi10.1021/acsami.2c20859
dc.identifier.issn1944-8252
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/34233
dc.relation.ispartofACS Applied Materials and Interfaces
dc.rightsAcesso Aberto
dc.subject.otherlanguageBacteria detection
dc.subject.otherlanguageBiosensing
dc.subject.otherlanguageFabrication
dc.subject.otherlanguageGraphene electrodes
dc.subject.otherlanguageNanomaterials
dc.titleLaser Reduced Graphene Oxide Electrode for Pathogenic Escherichia coli Detection
dc.typeArtigo
local.scopus.citations32
local.scopus.eid2-s2.0-85148416476
local.scopus.updated2024-12-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85148416476&origin=inward
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