Muscle IGF-1-induced skeletal muscle hypertrophy evokes higher insulin sensitivity and carbohydrate use as preferential energy substrate

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dc.contributor.authorChristoffolete M.A.
dc.contributor.authorSilva W.J.
dc.contributor.authorRamos G.V.
dc.contributor.authorBento M.R.
dc.contributor.authorCosta M.O.
dc.contributor.authorRibeiro M.O.
dc.contributor.authorOkamoto M.M.
dc.contributor.authorLohmann T.H.
dc.contributor.authorMachado U.F.
dc.contributor.authorMusaro A.
dc.contributor.authorMoriscot A.S.
dc.date.accessioned2024-03-13T00:59:34Z
dc.date.available2024-03-13T00:59:34Z
dc.date.issued2015
dc.description.abstract© 2015 Marcelo Augusto Christoffolete et al.We characterized the metabolic profile of transgenic mice exhibiting enhanced muscle mass driven by increased mIGF-1 expression (MLC/mIGF-1). As expected, 6-month-old MLC/mIGF-1 mice were heavier than age-matched wild type (WT) mice (37.4 ± 0.3 versus 31.8 ± 0.6 g, resp.). MLC/mIGF-1 mice had higher respiratory quotient when compared to WT (0.9 ± 0.03 versus 0.74 ± 0.02, resp.) suggesting a preference for carbohydrate as the major fuel source. MLC/mIGF-1 mice had a higher rate of glucose disposal when compared to WT (3.25 ± 0.14 versus 2.39 ± 0.03%/min, resp.). The higher disposal rate correlated to ~2-fold higher GLUT4 content in the extensor digitorum longus (EDL) muscle. Analysis of mRNA content for the glycolysis-related gene PFK-1 showed ~3-fold upregulation in MLC/mIGF-1 animals. We also found a 50% downregulation of PGC1α mRNA levels in MLC/mIGF-1 mouse EDL muscle, suggesting less abundant mitochondria in this tissue. We found no difference in the expression of PPARα and PPARβ/δ, suggesting no modulation of key elements in oxidative metabolism. These data together suggest a shift in metabolism towards higher carbohydrate utilization, and that could explain the increased insulin sensitivity of hypertrophied skeletal muscle in MLC/mIGF-1 mice.
dc.description.volume2015
dc.identifier.doi10.1155/2015/282984
dc.identifier.issn2314-6141
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36332
dc.relation.ispartofBioMed Research International
dc.rightsAcesso Aberto
dc.titleMuscle IGF-1-induced skeletal muscle hypertrophy evokes higher insulin sensitivity and carbohydrate use as preferential energy substrate
dc.typeArtigo
local.scopus.citations18
local.scopus.eid2-s2.0-84924025142
local.scopus.subjectAnimals
local.scopus.subjectCarbohydrate Metabolism
local.scopus.subjectGlucose Transporter Type 4
local.scopus.subjectHypertrophy
local.scopus.subjectInsulin
local.scopus.subjectInsulin Resistance
local.scopus.subjectInsulin-Like Growth Factor I
local.scopus.subjectMice
local.scopus.subjectMice, Transgenic
local.scopus.subjectMitochondria
local.scopus.subjectMuscle Proteins
local.scopus.subjectMuscle, Skeletal
local.scopus.subjectPeroxisome Proliferator-Activated Receptors
local.scopus.subjectRNA, Messenger
local.scopus.subjectTranscription Factors
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84924025142&origin=inward
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