Performance of collagen-based matrices from Nile tilapia skin: A pilot proteomic study in a murine model of wound healing

Página do item simplificado
dc.contributor.authorMedeiros C.B.A.
dc.contributor.authorde Lima I.L.
dc.contributor.authorCahu T.B.
dc.contributor.authorMuniz B.R.
dc.contributor.authorRibeiro M.H.M.L.
dc.contributor.authorde Carvalho E.H.
dc.contributor.authorEberlin M.N.
dc.contributor.authorMiranda M.J.B.
dc.contributor.authorde Souza Bezerra R.
dc.contributor.authorda Silva R.A.
dc.contributor.authorde Lima Filho J.L.
dc.date.accessioned2024-03-12T19:07:32Z
dc.date.available2024-03-12T19:07:32Z
dc.date.issued2024
dc.description.abstract© 2023 John Wiley & Sons, Ltd.Full-thickness cutaneous trauma, due to the lack of dermis, leads to difficulty in epithelialization by keratinocytes, developing a fibrotic scar, with less elasticity than the original skin, which may have disorders in predisposed individuals, resulting in hypertrophic scar and keloids. Biomedical materials have excellent characteristics, such as good biocompatibility and low immunogenicity, which can temporarily replace traditional materials used as primary dressings. In this work, we developed two dermal matrices based on Nile tilapia collagen, with (M_GAG) and without (M) glycosaminoglycans, using a sugarcane polymer membrane as a matrix support. To assess the molecular mechanisms driving wound healing, we performed qualitative proteomic analysis on the wound bed in an in vivo study involving immunocompetent murine models at 14 and 21 days post-full-thickness skin injury. Gene Ontology and Pathway analysis revealed that both skins were markedly represented by modulation of the immune system, emphasizing controlling the acute inflammation response at 14 and 21 days post-injury. Furthermore, both groups showed significant enrichment of pathways related to RNA and protein metabolism, suggesting an increase in protein synthesis required for tissue repair and proper wound closure. Other pathways, such as keratinization and vitamin D3 metabolism, were also enriched in the groups treated with M matrix. Finally, both matrices improved wound healing in a full post-thick skin lesion. However, our preliminary molecular data reveals that the collagen-mediated healing matrix lacking glycosaminoglycan (M) exhibited a phenotype more favorable to tissue repair, making it more suitable for use before skin grafts.
dc.description.issuenumber1
dc.description.volume59
dc.identifier.doi10.1002/jms.4988
dc.identifier.issn1096-9888
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/33969
dc.relation.ispartofJournal of Mass Spectrometry
dc.rightsAcesso Restrito
dc.subject.otherlanguagebiomaterial
dc.subject.otherlanguagecollagen
dc.subject.otherlanguageproteomics
dc.subject.otherlanguagetilapia
dc.subject.otherlanguagewound healing
dc.subject.otherlanguagexenograft
dc.titlePerformance of collagen-based matrices from Nile tilapia skin: A pilot proteomic study in a murine model of wound healing
dc.typeArtigo
local.scopus.citations0
local.scopus.eid2-s2.0-85180149170
local.scopus.subjectGlycosaminoglycans
local.scopus.subjectmatrix
local.scopus.subjectMurine model
local.scopus.subjectNile tilapia
local.scopus.subjectPerformance
local.scopus.subjectProteomics
local.scopus.subjectTilapia
local.scopus.subjectTissue repair
local.scopus.subjectWound healing
local.scopus.subjectXenograft
local.scopus.subjectAnimals
local.scopus.subjectCichlids
local.scopus.subjectCollagen
local.scopus.subjectDisease Models, Animal
local.scopus.subjectHumans
local.scopus.subjectMice
local.scopus.subjectProteomics
local.scopus.subjectWound Healing
local.scopus.updated2024-12-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85180149170&origin=inward
Arquivos
Coleções
Artigos de periódico