Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis

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dc.contributor.authorPaz-Cedeno F.R.
dc.contributor.authorCarceller J.M.
dc.contributor.authorIborra S.
dc.contributor.authorDonato R.K.
dc.contributor.authorGodoy A.P.
dc.contributor.authorVeloso de Paula A.
dc.contributor.authorMonti R.
dc.contributor.authorCorma A.
dc.contributor.authorMasarin F.
dc.date.accessioned2024-03-12T19:21:33Z
dc.date.available2024-03-12T19:21:33Z
dc.date.issued2021
dc.description.abstract© 2020 Elsevier LtdFor producing second-generation ethanol (cellulosic ethanol) and other value-added bioproducts, magnetic graphene oxide (GO-MNP) was synthesized in this work and used as the immobilization support for an industrial cellulase-and xylanase-containing preparation. GO-MNP characterization by TEM, SEM and ATR-FTIR spectroscopy showed that the magnetic nanoparticles are homogeneously distributed onto the GO sheets surface. The enzymatic preparation was immobilized by means of carbodiimide cross-linking chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (NHS). The supported final biocatalyst (GO-MNP-Enz) showed high activity for the hydrolysis of pretreated sugarcane bagasse (PSB) and presented relative endoglucanase, xylanase, β-glucosidase, and β-xylosidase activities of 70%, 66%, 88%, and 70%, respectively, after 10 cycles of hydrolysis of their respective substrates. The biocatalyst also maintained approximately 50% and 80% of its efficiency for cellulose and xylan hydrolysis, respectively, being the TOF (g.g−1.h−1) the highest observed when compared with previous results reported in literature. These findings suggest that GO-MNP-Enz may be a prospective candidate for industrial applications such as second-generation ethanol production.
dc.description.firstpage491
dc.description.lastpage501
dc.description.volume164
dc.identifier.doi10.1016/j.renene.2020.09.059
dc.identifier.issn1879-0682
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/34717
dc.relation.ispartofRenewable Energy
dc.rightsAcesso Restrito
dc.subject.otherlanguageBiocatalyst
dc.subject.otherlanguageEnzyme immobilization
dc.subject.otherlanguageGraphene oxide
dc.subject.otherlanguageMagnetic nanoparticles
dc.subject.otherlanguageMonomeric fermentable sugars
dc.subject.otherlanguageSugarcane bagasse hydrolysis
dc.titleMagnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis
dc.typeArtigo
local.scopus.citations56
local.scopus.eid2-s2.0-85091678529
local.scopus.subjectATR FT-IR spectroscopies
local.scopus.subjectCellulase and xylanase
local.scopus.subjectEnzymatic preparation
local.scopus.subjectEthanol production
local.scopus.subjectImmobilization support
local.scopus.subjectN-hydroxysuccinimide
local.scopus.subjectSugar-cane bagasse
local.scopus.subjectUltrastructural characterizations
local.scopus.updated2024-07-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85091678529&origin=inward
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