Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis
dc.contributor.author | Paz-Cedeno F.R. | |
dc.contributor.author | Carceller J.M. | |
dc.contributor.author | Iborra S. | |
dc.contributor.author | Donato R.K. | |
dc.contributor.author | Godoy A.P. | |
dc.contributor.author | Veloso de Paula A. | |
dc.contributor.author | Monti R. | |
dc.contributor.author | Corma A. | |
dc.contributor.author | Masarin F. | |
dc.date.accessioned | 2024-03-12T19:21:33Z | |
dc.date.available | 2024-03-12T19:21:33Z | |
dc.date.issued | 2021 | |
dc.description.abstract | © 2020 Elsevier LtdFor producing second-generation ethanol (cellulosic ethanol) and other value-added bioproducts, magnetic graphene oxide (GO-MNP) was synthesized in this work and used as the immobilization support for an industrial cellulase-and xylanase-containing preparation. GO-MNP characterization by TEM, SEM and ATR-FTIR spectroscopy showed that the magnetic nanoparticles are homogeneously distributed onto the GO sheets surface. The enzymatic preparation was immobilized by means of carbodiimide cross-linking chemistry using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (NHS). The supported final biocatalyst (GO-MNP-Enz) showed high activity for the hydrolysis of pretreated sugarcane bagasse (PSB) and presented relative endoglucanase, xylanase, β-glucosidase, and β-xylosidase activities of 70%, 66%, 88%, and 70%, respectively, after 10 cycles of hydrolysis of their respective substrates. The biocatalyst also maintained approximately 50% and 80% of its efficiency for cellulose and xylan hydrolysis, respectively, being the TOF (g.g−1.h−1) the highest observed when compared with previous results reported in literature. These findings suggest that GO-MNP-Enz may be a prospective candidate for industrial applications such as second-generation ethanol production. | |
dc.description.firstpage | 491 | |
dc.description.lastpage | 501 | |
dc.description.volume | 164 | |
dc.identifier.doi | 10.1016/j.renene.2020.09.059 | |
dc.identifier.issn | 1879-0682 | |
dc.identifier.uri | https://dspace.mackenzie.br/handle/10899/34717 | |
dc.relation.ispartof | Renewable Energy | |
dc.rights | Acesso Restrito | |
dc.subject.otherlanguage | Biocatalyst | |
dc.subject.otherlanguage | Enzyme immobilization | |
dc.subject.otherlanguage | Graphene oxide | |
dc.subject.otherlanguage | Magnetic nanoparticles | |
dc.subject.otherlanguage | Monomeric fermentable sugars | |
dc.subject.otherlanguage | Sugarcane bagasse hydrolysis | |
dc.title | Magnetic graphene oxide as a platform for the immobilization of cellulases and xylanases: Ultrastructural characterization and assessment of lignocellulosic biomass hydrolysis | |
dc.type | Artigo | |
local.scopus.citations | 56 | |
local.scopus.eid | 2-s2.0-85091678529 | |
local.scopus.subject | ATR FT-IR spectroscopies | |
local.scopus.subject | Cellulase and xylanase | |
local.scopus.subject | Enzymatic preparation | |
local.scopus.subject | Ethanol production | |
local.scopus.subject | Immobilization support | |
local.scopus.subject | N-hydroxysuccinimide | |
local.scopus.subject | Sugar-cane bagasse | |
local.scopus.subject | Ultrastructural characterizations | |
local.scopus.updated | 2024-07-01 | |
local.scopus.url | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85091678529&origin=inward |