Screen-Printed Nanohybrid Palladium-Based Electrodes for Fast and Simple Determination of Estradiol in Livestock

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dc.contributor.authorLopes C.S.C.
dc.contributor.authorSilva F.W.L.
dc.contributor.authorFernandes J.D.S.
dc.contributor.authorFernandes J.O.
dc.contributor.authorFerreira J.H.A.
dc.contributor.authorBrandao F.Z.
dc.contributor.authorSantelli R.E.
dc.contributor.authorCanevari T.C.
dc.contributor.authorCincotto F.H.
dc.date.accessioned2024-12-01T06:12:20Z
dc.date.available2024-12-01T06:12:20Z
dc.date.issued2024
dc.description.abstract© 2024 The Authors.One of the main challenges in animal breeding systems is determining estradiol (E2) in livestock samples as simple and minimally invasive as possible, Thus, a nonenzymatic biosensor screen-printed electrode (SPE) was developed by modifying nanohybrid palladium nanoparticles (PdNPs), and carbon dots anchored on a nanosilica particle (PdNPs/C.dots/SiO2), denominated SPE/PdNPs/C.dots/SiO2, and successfully tested for the direct detection of estradiol in livestock samples. PdNPs were directly obtained by a one-step synthesis through carbon dot reduction. Hybrid nanomaterials were characterized by atomic force microscopy, high-resolution transmission electron microscopy, and electrochemical impedance. The combination of PdNPs with C.dots resulted in a nonenzymatic biosensor supported on a screen-printed platform with superior electrocatalytic properties regarding the oxidation of E2 when compared to unmodified sensors. Modifications in the working electrode resulted in high sensitivity toward E2 determination within a linear range from 0.005 to 14.0 μmol L-1 with a limit of detection of 1.0 nmol L-1. The recovery rate of E2 in bovine serum samples and urine samples ranged from 92 to 106%. Interference studies showed that peak current variation (Δip) among all interferents evaluated and E2 did not exceed ±2%. The newly developed sensor stands out not only for its high sensitivity but also for its quick and simple way of production while also being disposable after analysis, providing a simple, sensitive, and practical approach for the determination of reproductive hormones in livestock.
dc.identifier.doi10.1021/acsomega.4c07861
dc.identifier.issnNone
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/39806
dc.relation.ispartofACS Omega
dc.rightsAcesso Aberto
dc.titleScreen-Printed Nanohybrid Palladium-Based Electrodes for Fast and Simple Determination of Estradiol in Livestock
dc.typeArtigo
local.scopus.citations0
local.scopus.eid2-s2.0-85208399015
local.scopus.updated2025-07-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85208399015&origin=inward
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