Leishmania amazonensis META2 protein confers protection against heat shock and oxidative stress

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dc.contributor.authorRamos C.S.
dc.contributor.authorYokoyama-Yasunaka J.K.U.
dc.contributor.authorGuerra-Giraldez C.
dc.contributor.authorPrice H.P.
dc.contributor.authorMortara R.A.
dc.contributor.authorSmith D.F.
dc.contributor.authorUliana S.R.B.
dc.date.accessioned2024-03-13T01:29:38Z
dc.date.available2024-03-13T01:29:38Z
dc.date.issued2011
dc.description.abstractThe META cluster of Leishmania amazonensis contains both META1 and META2 genes, which are upregulated in metacyclic promastigotes and encode proteins containing the META domain. Previous studies defined META2 as a 48.0-kDa protein, which is conserved in other Leishmania species and in Trypanosoma brucei. In this work, we demonstrate that META2 protein expression is regulated during the Leishmania life cycle but constitutive in T. brucei. META2 protein is present in the cytoplasm and flagellum of L. amazonensis promastigotes. Leishmania META2-null replacement mutants are more sensitive to oxidative stress and, upon heat shock, assume rounded morphology with shortened flagella. The increased susceptibility of null parasites to heat shock is reversed by extra-chromosomal expression of the META2 gene. Defective Leishmania promastigotes exhibit decreased ability to survive in macrophages. By contrast, META2 expression is decreased by 80% in RNAi-induced T. brucei bloodstream forms with no measurable effect on survival or resistance to heat shock. © 2010 Elsevier Inc.
dc.description.firstpage228
dc.description.issuenumber1
dc.description.lastpage237
dc.description.volume127
dc.identifier.doi10.1016/j.exppara.2010.08.004
dc.identifier.issn0014-4894
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37078
dc.relation.ispartofExperimental Parasitology
dc.rightsAcesso Aberto
dc.subject.otherlanguageCalpain-like
dc.subject.otherlanguageHeat shock
dc.subject.otherlanguageLeishmania amazonensis
dc.subject.otherlanguageMetacyclogenesis
dc.subject.otherlanguageOxidative stress
dc.subject.otherlanguageTrypanosoma brucei
dc.titleLeishmania amazonensis META2 protein confers protection against heat shock and oxidative stress
dc.typeArtigo
local.scopus.citations11
local.scopus.eid2-s2.0-78650555999
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78650555999&origin=inward
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