Bioassay-Guided Fractionation of Anti-Leishmania Amazonensis Extracts from Ampelozizyphus Amazonicus by High-Speed Countercurrent Chromatography

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dc.contributor.authorMendonca S.C.
dc.contributor.authorRangel de Paula L.
dc.contributor.authorSoares D.C.
dc.contributor.authordo Nascimento A.M.
dc.contributor.authorFreire Campos M.
dc.contributor.authorGomes B.A.
dc.contributor.authorda Fonseca T.S.
dc.contributor.authorSimas R.C.
dc.contributor.authorSaraiva E.M.
dc.contributor.authorLeitao S.G.
dc.contributor.authorLeitao G.G.
dc.date.accessioned2024-12-01T06:10:28Z
dc.date.available2024-12-01T06:10:28Z
dc.date.issued2024
dc.description.abstract© 2024 Wiley-VHCA AG, Zurich, Switzerland.Ampelozizyphus amazonicus Ducke, known as “saracura-mirá” in the Amazon rainforest, is valued for its traditional use in malaria prevention and treatment. The plant's roots and bark are employed for these purposes, while the wood is often overlooked. Given the global importance of leishmaniasis, this research focused on A. amazonicus anti-Leishmania amazonensis potential. The ethyl acetate extract from the bark (EAEB) exhibited the most effective inhibition of intracellular amastigote growth with IC50 7.0 μg mL−1 but showed high toxicity (CC50 9.0 μg mL−1). The wood ethanol (EW) and ethyl acetate (EAEW) extracts demonstrated promising results against L. amazonensis with IC50 15.8 μg mL−1 and 10.0 μg mL−1, CC50 50.5 μg mL−1 and 22.7 μg mL−1, respectively, with EW showing the greatest promise. High-speed countercurrent chromatography (HSCCC) combined with mass spectrometry (MS) off-line detection was employed for the bioassay-guided fractionation of EAEB and EAEW using HEMWat as a solvent system. These extracts showed to be rich in saponins and triterpenes, besides the presence of glycosylated flavonoids recently reported as minor compounds. The fractions obtained from HSCCC showed high toxicity. However, this technique proved effective in obtaining fractions with a high purity level, leading to the identification of a di-glycosylated triterpene saponin from EAEB, and melaleucic acid from EAEW. These findings highlight A. amazonicus as a potential source for developing new therapeutic alternatives for leishmaniasis and HSCCC as a technique enabling better separation efficiency of complex saponin matrices.
dc.description.issuenumber11
dc.description.volume21
dc.identifier.doi10.1002/cbdv.202400669
dc.identifier.issnNone
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/39789
dc.relation.ispartofChemistry and Biodiversity
dc.rightsAcesso Restrito
dc.subject.otherlanguageChalcone
dc.subject.otherlanguageDammarane saponins
dc.subject.otherlanguageLeishmania
dc.subject.otherlanguageRhamnaceae
dc.subject.otherlanguageSaracura-mirá
dc.titleBioassay-Guided Fractionation of Anti-Leishmania Amazonensis Extracts from Ampelozizyphus Amazonicus by High-Speed Countercurrent Chromatography
dc.typeArtigo
local.scopus.citations0
local.scopus.eid2-s2.0-85209631690
local.scopus.subjectAnimals
local.scopus.subjectAntiprotozoal Agents
local.scopus.subjectBiological Assay
local.scopus.subjectCountercurrent Distribution
local.scopus.subjectDose-Response Relationship, Drug
local.scopus.subjectLeishmania
local.scopus.subjectMice
local.scopus.subjectParasitic Sensitivity Tests
local.scopus.subjectPlant Bark
local.scopus.subjectPlant Extracts
local.scopus.updated2025-07-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85209631690&origin=inward
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