Cellular cadmium transport in gills and hepatopancreas of Ucides Cordatus, a mangrove crab

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dc.contributor.authorOrtega P.
dc.contributor.authorZanotto F.P.
dc.date.accessioned2024-03-13T01:06:39Z
dc.date.available2024-03-13T01:06:39Z
dc.date.issued2012
dc.description.abstractUcides cordatus is a mangrove crab, and has a very important role in human food consumption. Mangrove areas can be contaminated with heavy metals, like cadmium (Cd), through waste and disposal of batteries from industries. This metal reaches the animal through its gills, when is dissolved in the water, or through its hepatopancreas, from consumption of contaminated food. Because this metal does not have any physiological role for the animal, small concentrations can be extremely toxic. It is not known how cadmium enters the cells, but, because it is a divalent metal, it could enter cells together with calcium, using its plasma membrane channels to penetrate the cells. Therefore, the objective of the work was to characterize the kinetics of Cd transport. For this, the gill cells were separated by enzymatic dissociation, and the hepatopancreatic cells were dissociated by magnetic stirring, then, the cells were separated by sucrose gradient, and labeled with Fluo 3 AM. After that, the kinetics of Cd transport was characterized in the spectrofluorimeter, with addition of successive CdSO4 concentrations (0.5, 1.0 and 1.5 μM), respectively. Results showed a sigmoidal curve for Cd transport, suggesting that others ions, like calcium, for example, can participate in the transport of Cd. © 2012 by Nova Science Publishers, Inc. All rights reserved.
dc.description.firstpage107
dc.description.lastpage122
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36727
dc.relation.ispartofCrabs: Anatomy, Habitat and Ecological Significance
dc.rightsAcesso Restrito
dc.subject.otherlanguageCdso4
dc.subject.otherlanguageGills
dc.subject.otherlanguageHepatopancreas
dc.subject.otherlanguageTransport
dc.subject.otherlanguageUcides cordatus
dc.titleCellular cadmium transport in gills and hepatopancreas of Ucides Cordatus, a mangrove crab
dc.typeCapítulo de livro
local.scopus.citations1
local.scopus.eid2-s2.0-84892202462
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892202462&origin=inward
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