Antiviral, Cytoprotective, and Anti-Inflammatory Effect of Ampelozizyphus amazonicus Ducke Ethanolic Wood Extract on Chikungunya Virus Infection

Página do item simplificado
dc.contributor.authorRocha D.C.P.
dc.contributor.authorSisnande T.
dc.contributor.authorGavino-Leopoldino D.
dc.contributor.authorGuimaraes-Andrade I.P.
dc.contributor.authorCruz F.F.
dc.contributor.authorAssuncao-Miranda I.
dc.contributor.authorMendonca S.C.
dc.contributor.authorLeitao G.G.
dc.contributor.authorSimas R.C.
dc.contributor.authorMohana-Borges R.
dc.contributor.authorLeitao S.G.
dc.contributor.authorAllonso D.
dc.date.accessioned2024-03-12T19:08:15Z
dc.date.available2024-03-12T19:08:15Z
dc.date.issued2023
dc.description.abstract© 2023 by the authors.Chikungunya fever, a debilitating disease caused by Chikungunya virus (CHIKV), is characterized by a high fever of sudden onset and an intense arthralgia that impairs individual regular activities. Although most symptoms are self-limited, long-term persistent arthralgia is observed in 30–40% of infected individuals. Currently, there is no vaccine or specific treatment against CHIKV infection, so there is an urgent need for the discovery of new therapeutic options for CHIKF chronic cases. This present study aims to test the antiviral, cytoprotective, and anti-inflammatory activities of an ethanol extract (FF72) from Ampelozizyphus amazonicus Ducke wood, chemically characterized using mass spectrometry, which indicated the major presence of dammarane-type triterpenoid saponins. The major saponin in the extract, with a deprotonated molecule ion m/z 897 [M-H]−, was tentatively assigned as a jujubogenin triglycoside, a dammarane-type triterpenoid saponin. Treatment with FF72 resulted in a significant reduction in both virus replication and the production of infective virions in BHK-21-infected cells. The viability of infected cells was assessed using an MTT, and the result indicated that FF72 treatment was able to revert the toxicity mediated by CHIKV infection. In addition, FF72 had a direct effect on CHIKV, since the infectivity was completely abolished in the presence of the extract. FF72 treatment also reduced the expression of the major pro-inflammatory mediators overexpressed during CHIKV infection, such as IL-1β, IL-6, IL-8, and MCP-1. Overall, the present study elucidates the potential of FF72 to become a promising candidate of herbal medicine for alphaviruses infections.
dc.description.issuenumber11
dc.description.volume15
dc.identifier.doi10.3390/v15112232
dc.identifier.issn1999-4915
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/34006
dc.relation.ispartofViruses
dc.rightsAcesso Aberto
dc.subject.otherlanguageantivirals
dc.subject.otherlanguagechikungunya fever
dc.subject.otherlanguagechikungunya virus
dc.subject.otherlanguagejujubogenin triglycoside
dc.subject.otherlanguagesaponins
dc.subject.otherlanguagetriterpenoids
dc.titleAntiviral, Cytoprotective, and Anti-Inflammatory Effect of Ampelozizyphus amazonicus Ducke Ethanolic Wood Extract on Chikungunya Virus Infection
dc.typeArtigo
local.scopus.citations1
local.scopus.eid2-s2.0-85177748900
local.scopus.subjectAntiviral Agents
local.scopus.subjectArthralgia
local.scopus.subjectChikungunya Fever
local.scopus.subjectChikungunya virus
local.scopus.subjectEthanol
local.scopus.subjectHumans
local.scopus.subjectPlant Extracts
local.scopus.subjectSaponins
local.scopus.subjectTriterpenes
local.scopus.subjectVirus Replication
local.scopus.subjectWood
local.scopus.updated2024-12-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85177748900&origin=inward
Arquivos
Coleções
Artigos de periódico