Sulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom

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dc.contributor.authorPires C.L.
dc.contributor.authorRodrigues S.D.
dc.contributor.authorBristot D.
dc.contributor.authorGaeta H.H.
dc.contributor.authorToyama D.O.
dc.contributor.authorRonald W.
dc.contributor.authorFarias L.
dc.contributor.authorToyama M.H.
dc.date.accessioned2024-03-13T01:06:06Z
dc.date.available2024-03-13T01:06:06Z
dc.date.issued2013
dc.description.abstractSulfated polysaccharides derived from seaweed have shown great potential for use in the development of new drugs. In this study, we observed that a low-molecular-weight sulfated polysaccharide from Caulerpa racemosa, termed CrSP, could interact with secretory phospholipase A2 (sPLA2) isolated from Crotalus durissus terrifi cus venom. When native sPLA2 (14 kDa) was incubated with CrSP, they formed a molecular complex (sPLA2:CrSP) with a molecular mass of 32 kDa, approximately. Size exclusion chromatography experiments suggested that CrSP formed a stable complex with sPLA2. We belived that sPLA2 and SPCr are involved an ionic interaction between negatively charged CrSP and the positively charged basic amino acid residues of sPLA2, because this interaction induced signifi cant changes in sPLA2 enzymatic and pharmacological activities. CrSP caused a signifi cant increase in sPLA2 enzymatic and bactericidal activity and increased its edematogenic effect. A pharmacological assay showed that the myotoxic activity of sPLA2:CrSP is unrelated to its enzymatic activity and that sPLA2:CrSP may have a practical application as a natural antibacterial agent for use in humans and commercially raised animals.
dc.description.firstpage635
dc.description.issuenumber4
dc.description.lastpage643
dc.description.volume23
dc.identifier.doi10.1590/S0102-695X2013005000050
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36696
dc.relation.ispartofRevista Brasileira de Farmacognosia
dc.rightsAcesso Aberto
dc.subject.otherlanguageCrotalus durissus terrificus
dc.subject.otherlanguageEdematogenic effect
dc.subject.otherlanguageMyotoxic activity
dc.subject.otherlanguageSeaweed
dc.subject.otherlanguageSecretory phospholipase A2
dc.subject.otherlanguageSulfated polysaccharides
dc.titleSulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom
dc.typeArtigo
local.scopus.citations12
local.scopus.eid2-s2.0-84884694229
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84884694229&origin=inward
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