Machine-learning-assisted waveguide scattering microscopy for the immunological detection of bovine brucellosis: A proof concept
Tipo
Artigo
Data de publicação
2025
Periódico
Sensors and Actuators B: Chemical
Citações (Scopus)
0
Autores
Fonsaca J.E.S.
Teixeira W.S.R.
Tieppo B.
Raitz C.
Rehan M.
Gerosa R.M.
Megid J.
Appolinario C.M.
Salles M.O.
Saito L.A.M.
Vale D.L.
Grasseschi D.
de Matos C.J.S.
Teixeira W.S.R.
Tieppo B.
Raitz C.
Rehan M.
Gerosa R.M.
Megid J.
Appolinario C.M.
Salles M.O.
Saito L.A.M.
Vale D.L.
Grasseschi D.
de Matos C.J.S.
Orientador
Título da Revista
ISSN da Revista
Título de Volume
Membros da banca
Programa
Resumo
© 2025 Elsevier B.V.Photonic biosensors based on optical waveguides are at the forefront of biosensing technology, offering exceptional sensitivity and robustness. This study presents a proof-of-concept for detecting Brucella abortus antibodies in bovine serum using He-Ne laser-excited integrated optical waveguides. The antigen-antibody interactions in positive samples resulted in agglutination, forming scattering spots on the light-coupled waveguide, while no such spots were observed in negative samples. These spots were imaged over time using a microscope-coupled camera, generating 718 images that were processed to create a dataset for an artificial neural network (ANN). The ANN accurately distinguished between positive and negative samples, achieving 98.6 % accuracy, 98.7 % precision, and 98.7 % recall, with only a 1.4 % loss. This method detected bacterial antibodies in real animal samples within 20 min, using just 100 µL of reagents without requiring prior waveguide surface modification for antigen immobilization. Combining light scattering-based sensing protocols in photonic waveguides with machine learning tools offers a promising pathway for revolutionizing infectious disease diagnostics.
Descrição
Palavras-chave
Assuntos Scopus
Images processing , Immunological detection , Machine-learning , Negative samples , Neural-networks , Optical- , Photonic biosensor , Scattering microscopies , Silicon nitride waveguides , Waveguide scattering