Efficient electrochemical biosensors for ethynylestradiol based on the laccase enzyme supported on single walled carbon nanotubes decorated with nanocrystalline carbon quantum dots

Canevari T.C.; Cincotto F.H.; Nakamura M.; Machado S.A.S.; Toma H.E.

Efficient electrochemical biosensors for ethynylestradiol based on the laccase enzyme supported on single walled carbon nanotubes decorated with nanocrystalline carbon quantum dots

Tipo

Artigo

Data de publicação

2016

Periódico

Analytical Methods

Citações (Scopus)

22

Autores

Canevari T.C.
Cincotto F.H.
Nakamura M.
Machado S.A.S.
Toma H.E.

Resumo

© The Royal Society of Chemistry 2016.A new hybrid nanomaterial based on single-walled carbon nanotubes incorporating nanocrystalline carbon quantum dots (SWCNT/C-dots) was developed and characterized by means of high resolution transmission electron microscopy, atomic force microscopy, Raman spectroscopy and electrochemical techniques. A glassy carbon electrode modified with SWCNT/C-dots exhibited an enhanced electrocatalytic response for 17α-ethynylestradiol, reflecting an increase of the active area of the hybrid material, promoted by the C-dots. However, the best activity was observed for laccase immobilized on CGE/SWCNT/C-dots, suggesting that the nanocrystalline C-dots improve the electron transport between the substrate, SWCNTs and the copper ions in the enzyme active sites. Such an association provided a very efficient bioelectrochemical sensor for the 17α-ethynylestradiol endocrine interfering agent, with a detection limit of 4.0 nmol L-1 in real samples.

Assuntos Scopus

Bioelectrochemical sensors , Electrocatalytic response , Electrochemical biosensor , Electrochemical techniques , Enzyme active sites , Glassy carbon electrodes , Hybrid nanomaterials , Nanocrystalline carbon