Effects of Crotalus durissus collilineatus venom in the isolated rat kidney

dc.contributor.authorAmora D.N.
dc.contributor.authorSousa T.M.
dc.contributor.authorMartins A.M.C.
dc.contributor.authorBarbosa P.S.F.
dc.contributor.authorMagalhaes M.R.
dc.contributor.authorToyama M.H.
dc.contributor.authorFonteles M.C.
dc.contributor.authorMenezes D.B.D.
dc.contributor.authorMonteiro H.S.A.
dc.date.accessioned2024-03-13T01:42:39Z
dc.date.available2024-03-13T01:42:39Z
dc.date.issued2006
dc.description.abstractOphidian accidents caused by the subspecies Crotalus durissus are responsible for high morbity and mortality rates. Acute renal failure is a common complication observed in these accidents. The aim of the present study was to investigate the renal effects promoted by the venom of C. d. collilineatus and its fractions, crotoxin and phospholipase A2. C. d. collilineatus (Cdc; 30 μg mL-1), crotoxin (CTX; 10 μg mL -1) and phospholipase A2 (PLA2; 10 μg mL-1) were tested in isolated rat kidney. The first 30 min of each experiment were used as an internal control and Cdc or its fractions, CTX and PLA2 were added to the system after this period. All experiments lasted 120 min. The venom of Cdc decreased perfusion pressure (PP; control 120=110.3±3.69 mmHg; Cdc120=96.7±8.1 mmHg), renal vascular resistance (RVR; control120=6.42±0.78 mmHg mL g-1 min-1; Cdc120=4.8±0.56 mmHg/mL g-1 min-1), urinary flow (UF; control120=0. 19±0.03 mL g-1 min-1; Cdc120=0. 12±0.01 mL g-1 min-1), and glomerular filtration rate (GFR; control120=0.79±0.07 mL g-1 min -1; Cdc120=0.53±0.09 mL g-1 min -1), but had no effect on the percent of sodium tubular transport (%TNa+), percent of chloride tubular transport (%TK+) and percent of potassium tubular transport (%TCl-). CTX and PLA 2 reduced the GFR, while UF, PP and RVR remained stable during the full 120 min of perfusion. Crotoxin administration also diminished the %TK + (control120=69.94±6.49; CTX120=33. 28±4.78) and %TCl- (control120=79.53±2.67; CTX120=64.62±6.93). PLA2 reduced the %TK +, but exerted no effect on the %TNa+ or on that of TCl-. In conclusion, the C. d. collilineatus venom altered the renal functional parameters evaluated. We suggest that crotoxin and phospholipase A2 were involved in this process, since the renal effects observed would be due to the synergistic action of the components of the venom. © 2005 Elsevier Ltd. All rights reserved.
dc.description.firstpage260
dc.description.issuenumber3
dc.description.lastpage264
dc.description.volume47
dc.identifier.doi10.1016/j.toxicon.2005.10.007
dc.identifier.issn0041-0101
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37798
dc.relation.ispartofToxicon
dc.rightsAcesso Restrito
dc.subject.otherlanguageC.d. collilineatus
dc.subject.otherlanguageKidney
dc.subject.otherlanguageVenom
dc.titleEffects of Crotalus durissus collilineatus venom in the isolated rat kidney
dc.typeArtigo
local.scopus.citations14
local.scopus.eid2-s2.0-33644536114
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33644536114&origin=inward
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