Enzymatic and structural characterization of new PLA2 isoform isolated from white venom of Crotalus durissus ruruima

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dc.contributor.authorDiz Filho E.B.S.
dc.contributor.authorMarangoni S.
dc.contributor.authorToyama D.O.
dc.contributor.authorFagundes F.H.R.
dc.contributor.authorOliveira S.C.B.
dc.contributor.authorFonseca F.V.
dc.contributor.authorCalgarotto A.K.
dc.contributor.authorJoazeiro P.P.
dc.contributor.authorToyama M.H.
dc.date.accessioned2024-03-13T01:36:23Z
dc.date.available2024-03-13T01:36:23Z
dc.date.issued2009
dc.description.abstractThis work reports the structural and enzymatic characterization of a new sPLA2 from the white venom of Crotalus durissus ruruima, nominated PLA2A. The homogeneity of the PLA2A fraction and its molecular mass were initially evaluated by SDS-PAGE and confirmed by MALDI-TOF spectrometry, indicating a molecular mass of 14,299.34 Da. Structural investigation, through circular dichroism spectroscopy, revealed that PLA2A has a high content of alpha helix and beta-turn structures, 45.7% and 35.6% respectively. Its amino acid sequence, determined by Edman degradation and "de novo amino acid sequencing", exhibited high identity to PLA2 Cdt F15 from Crotalus durissus terrificus. The enzymatic investigation, conducted using the synthetic substrate 4-nitro-3-(octanoyloxy)-benzoic acid, determined its Vmax (7.56 nmoles/min) and KM (2.76 mM). Moreover, PLA2A showed an allosteric behavior and its enzymatic activity was dependent on Ca2+. Intrinsic fluorescence measurements suggested that Ca2+ induced a significant increase of PLA2A fluorescence, whereas its replacement for Mg2+, Mn2+, Sn2+ and Cd2+ apparently induced no structural modifications. The optimal pH and temperature for the enzymatic activity of PLA2A were 8.4 and 40 °C, respectively, and the minimal concentration of p-BPB and crotapotin that significantly inhibited such activity was 0.75 mM and 0.4 μM, respectively. In addition, PLA2A showed a significant antibacterial effect that was not strictly dependent on the enzymatic activity of such sPLA2. © 2008 Elsevier Ltd. All rights reserved.
dc.description.firstpage104
dc.description.issuenumber1
dc.description.lastpage114
dc.description.volume53
dc.identifier.doi10.1016/j.toxicon.2008.10.021
dc.identifier.issn0041-0101
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37455
dc.relation.ispartofToxicon
dc.rightsAcesso Restrito
dc.subject.otherlanguageCalcium binding
dc.subject.otherlanguageCalcium binding loop
dc.subject.otherlanguageCrotalus durissus ruruima
dc.subject.otherlanguagePLA2
dc.subject.otherlanguageVenom
dc.titleEnzymatic and structural characterization of new PLA2 isoform isolated from white venom of Crotalus durissus ruruima
dc.typeArtigo
local.scopus.citations34
local.scopus.eid2-s2.0-57849150819
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=57849150819&origin=inward
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