Pharmacokinetics, Pharmacodynamic Efficacy Prediction Indexes and Monte Carlo Simulations of Enrofloxacin Hydrochloride Against Bacterial Strains That Induce Common Clinical Diseases in Broiler Chickens

dc.contributor.authorBonassa K.P.D.
dc.contributor.authorMiragliotta M.Y.
dc.contributor.authorSimas R.C.
dc.contributor.authorEberlin M.N.
dc.contributor.authorAnadon A.
dc.contributor.authorMoreno R.A.
dc.contributor.authorReyes F.G.R.
dc.date.accessioned2024-03-12T19:21:40Z
dc.date.available2024-03-12T19:21:40Z
dc.date.issued2021
dc.description.abstract© Copyright © 2021 Bonassa, Miragliotta, Simas, Eberlin, Anadón, Moreno and Reyes.Pharmacokinetic parameters and efficacy prediction indexes (Cmax/MIC90 and AUC0−24/MIC90) of an enrofloxacin hydrochloride (ENR-HCl) veterinary product soluble in water were determined in healthy broiler chickens of both sexes after a single oral dose of ENR-HCl (equivalent to 10 mg ENR base/kg bw). Monte Carlo simulations targeting Cmax/MIC90 = 10 and AUC0−24/MIC90 =125 were also performed based on a set of MIC (minimum inhibitory concentration) values of bacterial strains that induce common clinical diseases in broiler chickens and that showed to be susceptible to ENR-HCl. Plasma concentrations of ENR and its main metabolite ciprofloxacin (CIP) were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Plasma concentration-time curves were found to fit a non-compartmental open model. The ratio of the area under the plasma concentration-time curve (AUC) of CIP/ENR was 4.91%. Maximum plasma concentrations of 1.35 ± 0.15 μg/mL for ENR-HCl and 0.09 ± 0.01 μg/mL for CIP were reached at 4.00 ± 0.00 h and 3.44 ± 1.01 h, respectively. Areas under the plasma vs. time concentration curve in 24 h (AUC0−24) were 18.91 ± 1.91 h × μg/mL and 1.19 ± 0.12 h × μg/mL for ENR-HCl and CIP, respectively. Using a microbroth dilution method, the minimum inhibitory concentration (MIC90) values were determined for ENR-HCl for 10 bacterial strains (Mycoplasma gallisepticum, Mycoplasma synoviae, Avibacterium paragallinarum, Clostridium perfringens, Escherichia coli, Pseudomonas aeruginosa, Salmonella ser. Enteritidis, Salmonella ser. Gallinarum, Salmonella ser. Pullorum, and Salmonella ser. Typhimurium), which are the most common causes of infectious clinical diseases in broiler chickens. In summary, the PK/PD ratios and Monte Carlo simulation were carried out for ENR-HCl in poultry, which due to its solubility was administered in drinking water. The PK/PD efficacy prediction indexes and Monte Carlo simulations indicated that the ENR-HCl oral dose used in this study is useful for bacterial infections in treating C. perfringens (Gram-positive), E. coli and S. ser. Enteritidis (Gram-negative) and M. gallisepticum bacteria responsible for systemic infections in poultry, predicting a success rate of 100% when MIC ≤ 0.06 μg/mL for E. coli and S. ser. Enteritidis and MIC ≤ 0.1 μg/mL for M. gallisepticum. For C. perfringens, the success rate was 98.26% for MIC ≤ 0.12. However, clinical trials are needed to confirm this recommendation.
dc.description.volume7
dc.identifier.doi10.3389/fvets.2020.606872
dc.identifier.issn2297-1769
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/34723
dc.relation.ispartofFrontiers in Veterinary Science
dc.rightsAcesso Aberto
dc.subject.otherlanguagebroiler chicken
dc.subject.otherlanguageciprofloxacin
dc.subject.otherlanguageenrofloxacin hydrochloride
dc.subject.otherlanguageMIC values
dc.subject.otherlanguageMonte Carlo simulations
dc.subject.otherlanguagepharmacodynamics
dc.subject.otherlanguagepharmacokinetics
dc.subject.otherlanguagePK/PD modeling
dc.titlePharmacokinetics, Pharmacodynamic Efficacy Prediction Indexes and Monte Carlo Simulations of Enrofloxacin Hydrochloride Against Bacterial Strains That Induce Common Clinical Diseases in Broiler Chickens
dc.typeArtigo
local.scopus.citations3
local.scopus.eid2-s2.0-85099726337
local.scopus.updated2024-06-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85099726337&origin=inward
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