The extract of the jellyfish Phyllorhiza punctata promotes neurotoxic effects

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dc.contributor.authorCarneiro R.F.V.
dc.contributor.authorNascimento N.R.F.D.
dc.contributor.authorCosta P.P.C.
dc.contributor.authorGomes V.M.
dc.contributor.authorde Souza A.J.F.
dc.contributor.authorde Oliveira S.C.B.
dc.contributor.authordos Santos Diz Filho E.B.
dc.contributor.authorZara F.J.
dc.contributor.authorFonteles M.C.
dc.contributor.authorde Oliveira Toyama D.
dc.contributor.authorToyama M.H.
dc.contributor.authorSantos C.F.
dc.date.accessioned2024-03-13T01:10:13Z
dc.date.available2024-03-13T01:10:13Z
dc.date.issued2011
dc.description.abstractPhyllorhiza punctata (P. punctata) is a jellyfish native to the southwestern Pacific. Herewith we present the biochemical and pharmacological characterization of an extract of the tentacles of P. punctata. The tentacles were subjected to three freeze-thaw cycles, homogenized, ultrafiltered, precipitated, centrifuged and lyophilized to obtain a crude extract (PHY-N). Paralytic shellfish poisoning compounds such as saxitoxin, gonyautoxin-4, tetrodotoxin and brevetoxin-2, as well as several secretory phospholipase A 2 were identified. PHY-N was tested on autonomic and somatic neuromuscular preparations. In mouse vas deferens, PHY-N induced phasic contractions that reached a peak of 234±34.7% of control twitch height, which were blocked with either 100μ m of phentolamine or 1m m of lidocaine. In mouse corpora cavernosa, PHY-N evoked a relaxation response, which was blocked with either L-N G-Nitroarginine methyl ester (0.5m m) or 1m m of lidocaine. PHY-N (1, 3 and 10μg ml -1) induced an increase in tonus of the biventer-cervicis neuromuscular preparation that was blocked with pre-treatment of galamine (10μ m). Administration of 6mg kg -1 PHY-N intramuscularly produced death in broilers by spastic paralysis. In conclusion, PHY-N induces nerve depolarization and nonspecifically increases neurotransmitter release. © 2011 John Wiley & Sons, Ltd.
dc.description.firstpage720
dc.description.issuenumber8
dc.description.lastpage729
dc.description.volume31
dc.identifier.doi10.1002/jat.1620
dc.identifier.issn1099-1263
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/36929
dc.relation.ispartofJournal of Applied Toxicology
dc.rightsAcesso Restrito
dc.subject.otherlanguageMarine neurotoxic alkaloids
dc.subject.otherlanguageNeuromuscular blockade
dc.subject.otherlanguageNeurotoxicity
dc.subject.otherlanguageNitrergic stimulation
dc.subject.otherlanguageSmooth muscle spasm
dc.subject.otherlanguageSpastic paralysis
dc.titleThe extract of the jellyfish Phyllorhiza punctata promotes neurotoxic effects
dc.typeArtigo
local.scopus.citations14
local.scopus.eid2-s2.0-81455143951
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=81455143951&origin=inward
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