Purification and biological activity of the thrombin-like substance isolated from Bothrops insularis venom

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dc.contributor.authorBraga M.D.M.
dc.contributor.authorMartins A.M.C.
dc.contributor.authorde Menezes D.B.
dc.contributor.authorBarbosa P.S.F.
dc.contributor.authorEvangelista J.S.A.M.
dc.contributor.authorToyama M.H.
dc.contributor.authorToyama D.O.
dc.contributor.authorFonteles M.C.
dc.contributor.authorMonteiro H.S.A.
dc.date.accessioned2024-03-13T01:40:10Z
dc.date.available2024-03-13T01:40:10Z
dc.date.issued2007
dc.description.abstractThe venom of Bothrops insularis snake, known in Brazil as jararaca ilhoa, contains a variety of proteolytic enzymes such as a thrombin-like substance that is responsible for various pharmacological effects. B. insularis venom chromatography profile showed an elution of seven main fractions. The thrombin-like activity was detected in fractions I and III, the latter being subjected to two other chromatographic procedures, so to say DEAE and Hi Trap Benzamidine. The purity degree of this fraction was confirmed by analytical reverse phase HPLC, which displayed only one main fraction confirmed by SDS-PAGE constituting fraction III. About 5 μg of fraction III protein potentiated the secretion of insulin induced by 2.8 mM of glucose in rats isolated pancreatic β-cells treated; the increase being around 3-fold higher than its respective control. B. insularis lectin (BiLec; 10 μg/mL) was also studied as to its effect on the renal function of isolated perfused rat kidneys with the use of six Wistar rats. BiLec increased perfusion pressure (PP), renal vascular resistence (RVR), urinary flow (UF) and glomerular filtration rate (GFR). Sodium (%TNa+) and chloride tubular reabsorption (%TCl-) decreased at 120 min, without alteration in potassium transport. In conclusion, the thrombin-like substance isolated from B. insularis venom induced an increase in insulin secretion, in vitro, and transiently altered vascular, glomerular and tubular parameters in the isolated rat kidney. © 2006 Elsevier Ltd. All rights reserved.
dc.description.firstpage329
dc.description.issuenumber3
dc.description.lastpage338
dc.description.volume49
dc.identifier.doi10.1016/j.toxicon.2006.10.005
dc.identifier.issn0041-0101
dc.identifier.urihttps://dspace.mackenzie.br/handle/10899/37664
dc.relation.ispartofToxicon
dc.rightsAcesso Restrito
dc.subject.otherlanguageBiological activity
dc.subject.otherlanguageBothrops insularis
dc.subject.otherlanguageRenal activity
dc.subject.otherlanguageThrombin-like substance
dc.titlePurification and biological activity of the thrombin-like substance isolated from Bothrops insularis venom
dc.typeArtigo
local.scopus.citations6
local.scopus.eid2-s2.0-33846585382
local.scopus.updated2024-05-01
local.scopus.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33846585382&origin=inward
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